SCF/c-Kit signaling promotes invasion of T24 cells via PI3K pathway
GUO Shujun
TAO Xiangnan
WANG Yimeng
TANG Jie
SHEN Lin
SONG Chuanwang
Abstract：Objective To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells. Methods Western blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24 cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was evaluated using Transwell invasion assay (direct and indirect counting methods). Results T24 cells expressed c-Kit protein and showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect was blocked by the application of Wortmannin before the stimulation. Conclusion SCF/c-Kit signaling promotes the invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.
Keywords：SCF/c-Kit signalingT24 cellsinvasionbladder cancer
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 507-510 )