Feasibility of continuous extracorporeal normothermic liver perfusion using autologous blood:a study in pigs
XIANG Kaimin
YU Huaxu
YUAN Jinzhong
LI Zheng
Abstract：Objective To study the feasibility of sustaining the viable status of a liver graft in at least 96 h by extracorporeal perfusion using autologous blood. Methods Eight extracorporeal porcine liver perfusions using autologous blood were performed, each for 96 h with hepatectomy, cold preservation, cannulation of vessels, and initiation of perfusion with normothermic oxygenated porcine blood. The graft viability was assessed by metabolic, synthetic, hemodynamic, and histologic parameters. Results After 96 h of normothermic, extracorporeal perfusion using autologous blood, the isolated livers maintained normal physiological levels of pH and electrolytes with sustained hepatic protein synthesis (complement and factor V) throughout the perfusion. Hemodynamic parameters maintained normal physiological ranges. Histological inspection demonstrated good preservation of the liver with a good architectural integrity. Conclusion It is possible to sustain the viable status of a liver graft within 96 h by extracorporeal perfusion using autologous blood.
Keywords：porcine liverautologous bloodextracorporeal perfusionhepatic failureorgan preservation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 223-227 )