Effects of pioglitazone on myocardial peroxisome proliferator-activated receptor gamma co-activator lαexpression in rats with myocardial ischemia/reperfusion injury
SHEN Lin
WANG Hao
YE Ping
Abstract：Objective To investigate the effects of pioglitazone on the expression of peroxisome proliferator-activated receptor gamma co-activator lα(PGC-lα) in rat myocardium following myocardial ischemia/reperfusion (I/R) injury. Methods Twenty-four SD rats were randomly divided into 4 equal groups, namely I/R group, pioglitazone (5 mg/kg daily) group, pioglitazone (10 mg/kg daily) group, and pioglitazone (10 mg/kg) +peroxisome proliferator-activated receptor γ (PPARγ)-specific antagonist GW9662 group. Myocardial I/R injury was induced by ligation of the left anterior descending coronary artery for 30 min and reperfusion for 120 min. Myocardial apoptosis following I/R injury was examined with TUNEL assay;RT-PCR and Western blotting were employed to detect the expression of PGC-lα mRNA and protein, respectively. Results Pioglitazone treatment significantly suppressed myocardial apoptosis (21.4%±8.8%、17.3%±8.7%、40.1%±12.3%, P<0.05) following I/R injury and up-regulated myocardial PGC-lα expression at both the mRNA and protein levels (P<0.05), but these effects were antagonized by GW9662 (P<0.05). Conclusion Pioglitazone can inhibit myocardial apoptosis induced by I/R injury and up-regulate myocardial PGC-lαexpression, and these effects are mediated by PPARγ.
Keywords：myocardiumischemia/reperfusion injurypioglitazoneapoptosisperoxisome proliferator-activated receptor gamma co-activator lα
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 197-200 )