Renal expression of RANK- RANKL in a rat model of puromycin aminonucleoside nephropathy
FENG Zhonglin
LIU Shuangxin
SHI Wei
XIAO Houqin
LIANG Xinling
LIU Xiaoying
YE Zhiming
WANG Suxia
LIANG Yongzheng
ZHANG Bin
WANG Wenjian
LIU Yanhui
MEI Ping
XU Lixia
MA Jianchao
XIA Yunfeng
Abstract：Objective To investigate RANK-RANKL expression in the kidneys of a rat model of puromycin aminonucleoside nephropathy (PAN). Methods Thirty-six SD rats were randomly divided into PAN model group and normal control group. PAN was induced by a single intravenous injection of 100 mg/kg puromycin aminonucleoside. Serum creatinine and 24-hour urinary protein were measured on days 3, 7, and 14 after the injection, and renal pathologies were assessed with optical and immune transmission electron microscopy. The expression of RANK and RANKL in the kidneys was examined using reverse transcription-ploymerase chain reaction (RT-PCR) and Western blotting. Results The PAN model rats showed massive proteinuria and elevated serum creatinine on day 3, which peaked on day 7. RANK-RANKL protein and mRNA expressions in PAN model group was higher than those in the control group. In the PAN rats, RANK was expressed mainly on the top cell membrane and in the cytoplasm of renal podocytes with a significantly increased expression level compared with that in the control group. Conclusion The PAN rat model shows aberrant RANK and RANKL expressions in the podocytes, indicating their contribution to podocyte injury in PAN.
Keywords：puromycin aminonucleoside nephropathykidneyspokocytesRANKRANKLmodelanimals
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 65-69 )