miR-124a promotes neurite outgrowth by inhibiting iASPP expression
LIN Lifang
GU Xi
LIU Shuhu
WANG Xuemin
Abstract：Objective To investigate the role of iASPP as the target gene of miR-124a in neural development. Methods Using the online bioinformatical tool (TargetScan) and by reviewing the relevant studies, we selected iASPP as the candidate target gene of miR-124a involved in early-stage neuronal differentiation. Luciferase reporter assay was used to verify the candidate gene. We transfected M17 cells with a miR-124a overexpression plasmid and detected the changes in the protein expression of iASPP using Western blotting. With retinoic acid-induced M17 cells as the neuronal differentiation model, the role of iASPP in early-stage neuronal differentiation was investigated by gene overexpression and gene interference techniques. Results miR-124a inhibited the expression of iASPP in M17 cells by interacting with the 3'UTR of iASPP gene. miR-124a promoted neurite outgrowth of the cells, which was blocked by iASPP overexpression. Conclusion miR-124a promotes neurite outgrowth of M17 cells by inhibiting iASPP expression.
Keywords：miR-124aiASPPneural developmentneurite outgrowth
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 31-35 )