Application of genome-wide genechip for screening and identifying genes related to CD133+CD200+colorectal cancer stem cells
ZHANG Shanshan
LI Lixuan
HUANG Zaiwei
XIN Xiaomin
XIAO Bing
Abstract：Objective To screen and identity genes related to CD133+CD200+ colorectal cancer stem cells. Methods The two subpopulations of colorectal cancer cells, namely CD133+CD200+ and CD133-CD200- cells, were sorted and verified by flow cytometry. The gene expression profiles of CD133 +CD200 +and CD133-CD200- colorectal cancer cells were examined using Affymetrix Human U133 Plus2.0 genome- wide genechip. The differentially expressed genes between the two cell subpopulations were analyzed to identify the genes responsible for the main effect in association with colorectal cancer stem cells. Real-time quantitative PCR was performed to confirm some of the differentially expressed genes identified by genechip. Results The genechip result showed that 655 genes were differentially expressed in CD133+CD200+colorectal cancer stem cells by at least 3 folds, including 290 up-regulated and 365 down-regulated ones. Bioinformatics analysis and gene co-expression network building identified 3 genes (MDM2, PRKACG, and CACNA1G) with specific expression in CD133+CD200+colorectal cancer stem cells, and this result was confirmed by real-time quantitative PCR analysis. Conclusion A specific gene expression profile of colorectal cancer stem cells has been established through screening and identifying genes related to CD133+CD200+colorectal cancer stem cells by gene genechip technique, which provides a basis for further study of gene targeting therapy of colorectal cancer.
Keywords：CD133CD200colorectal cancer stem cellsgenechipMAPK signaling pathway
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1787-1791 )