Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB
XIAO Yi
GAO Kun
YANG Yong
YAN Jinqi
ZHANG Liang
WANG Yu
XU Yuanji
TIAN Renli
DU Zhiyan
YU Jiyun
Abstract：Objective To investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX-tG250FcGB. Methods The DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared. Results The vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection. Conclusion Electroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.
Keywords：kidney neoplasmsDNA vaccineelectroporationimmune effect
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1628-1631 )