Screening molecular markers in early breast cancer of the same pathological types but with different prognoses using Agilent gene chip
LI Zhou
PENG Liang
HAN Shuai
HUANG Zonghai
SHI Fujun
CAI Zhai
LI Xiuqin
ZHANG Pusheng
ZHU Huijuan
JIN Weirong
Abstract：Objective:To screen molecular markers in early breast cancer and establish gene subtyping-based diagnostic criteria for predicting the prognosis of early breast cancers. Methods Tumor tissue specimens were obtained from 8 patients with early breast cancer for analysis of the differentially expressed genes using Agilent custom 8 × 15 000 chips in combination with the prognostic data of the patients. Another 42 tumor tissue specimens were used to validate the differential genes by real-time fluorescent quantitative PCR. Results Gene microarray analysis identified 132 differentially expressed genes between the patients with favorable and poor prognosis, and 44 of these genes were significantly up-regulated (by over two folds) and 88 down-regulated in patients with poor prognoses. Conclusion The gene expression profiles differ in early breast cancer tissues of the same pathological type but with different clinical stages and prognoses, and CD44, MKI67, NTRK2, Nek2, C16orf60, TOP2A, ANCCA, and RRM2 genes can be used as the prognostic markers for early breast cancer.
Keywords：breast tumorsgeneschip analysis techniquesmolecular typing
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1483-1488 )