Effects of peroxisome proliferator-activated receptorsγon the expression of insulin receptor substrate-4 gene in rat cortical neurons and mouse brain
ZHANG Hongyan
MENG Siying
LIN Lifang
WU Qiaoqi
ZHOU Riyang
WANG Xuemin
Abstract：Objective To investigate the effect of peroxisome proliferator-activated receptors γ (PPARγ) on insulin receptor substrate-4 (IRS-4) gene expression in the brain. Methods Primarily cultured cortical neurons from E17-18 Sprague Dawley rats, after 1 week of plating, were exposed to 10μmol/L PPARγagonist rosiglitazone for 0, 1, 4 or 24 h. Adult C57BL/6J mice or conditional brain PPARγ knock-out mice (B-PPARγ-KO, BKO) received an intraperitoneal injection of rosiglitazone in 10%DMSO at 12 mg/kg or injection of the same volume of saline containing 10%DMSO. The effect of rosiglitazone on the survival of the neurons was examined by MTT assay. The expression of IRS-4 mRNA was analyzed by real-time quantitative PCR. Results The survival of the cortical neurons showed no significant difference between the agonist groups and the control group. The expression of IRS-4 mRNA was significantly up-regulated in the cortical tissues and neurons of the agonist groups compared with the control groups (P<0.05), but in BKO mice without treatment, IRS-4 mRNA expression was significantly down-regulated (P<0.05). Conclusion PPARγcan enhance the expression of IRS-4 mRNA in the brain.
Keywords：cortexrosiglitazoneperoxisome proliferator-activated receptorsγinsulin receptor substrate-4
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1463-1466 )