Diammonium glycyrrhizinate inhibits airway smooth muscle proliferation in a murine mod-el of chronic asthma
XIAO Min
ZHU Tao
WANG Tao
WEN Fuqiang
Abstract：Objective To investigate the therapeutic value and possible mechanism of diammonium glycyrrhizinate (DG) in treatment of airway remodeling in a murine model of chronic asthma. Methods Thirty male BALB/C mice were randomly divided into control group, OVA+DG group and OVA group (n=10). HE staining was used to observe the pathological changes, and Masson's staining was used to detect and measure collagen deposition. Alpha-SMA and PPARγmRNA expressions were analyzed by RT-PCR, and the protein expressions ofα-SMA and PPARγwere measured by Western blotting. Results After 75 days of OVA sensitization and challenge, obvious pathological changes occurred in the lung tissues, which was more severe in OVA group than in OVA+DG group. Collagen deposition was significantly increased after OVA stimulation, but was obviously milder in OVA+DG group than in OVA group. OVA-induced up-regulation ofα-SMA was notably attenuated by DG injection. The expression of PPARγ was markedly down-regulated after OVA stimulation but was substantially enhanced after DG intervention. Conclusion DG can inhibit airway smooth muscle proliferation possibly through up-regulation of PPARγ in a murine model of chronic asthma.
Keywords：diammonium glycyrrhizinateasthmaairway smooth muscleperoxisome proliferators-activated receptor gam-maα-SMA
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1416-1420 )