Effect of the C-terminal truncated human apoptosis-inducing factorΔ1-480 on biological behaviors of MCF-7 cells
WANG Lijuan
XIAO Huawei
BAI E
ZHOU Xia
LI Meng
JIN Guihua
ZHU Qing
Abstract：Objective To observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells. Methods PcDNA3.0-FDT-AIFΔ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamins. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement. Results PcDNA3.0-FDT-AIFΔ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFΔ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05). Conclusion The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.
Keywords：C-terminal truncated human apoptosis-inducing factorΔ1-480MCF-7 cellsproliferationapoptosiscytochrome c
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1285-1288 )