Fenofibrate inhibits tumor necrosis factor-α-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells
LIN Rong
LIU Jun-tian
GAN Wei-jie
WANG Wei-rong
HAN Chun-jie
LIU Yu
FANG Zhi-yuan
Abstract：Objective To investigate the regulatory effects of fenofibrate on TNF-α-induced CD40 expression and matrix metalloproteinase (MMP) activity in human vascular endothelial cells (HUVECs). Methods Quantitative RT-PCR and flow cytometry were employed to evaluate the effect of fenofibrate on TNF-α-induced CD40 mRNA and cell surface CD40 expression in HUVECs, and gelatin zymography was used to determine the effect of fenofibrate on the gelatinolytic activities of MMP-2 and MMP-9 in TNF-α-stimulated HUVECs. Results Fenofibrate at the concentrations of5×10-5, 1×10-4 and 2×10-4mol/L significantly reduced TNF-α-induced increment of CD40 mRNA and cell surface CD40 expressions (P＜0.01), with the maximal inhibition achieved at the concentration of 1 × 10-4 mol/L. Fenofibrate at 2× 10-4 mol/L did not further decrease CD40expression induced by TNF-α. Fenofibrate significantly inhibited the stimulatory effect of TNF-α on MMP-2 and MMP-9 activities in HUVECs. Conclusion Fenofibrate reduces TNF-α-induced increment of CD40 expression and MMP-2 and MMP-9 activities in HUVECs.
Keywords：fenofibrateCD40matrix metalloproteinase-2matrix metalloproteinase-9
Publication Date：2006-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1383-1387 )