Preparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein
Abstract：Objective To prepare and characterize monoclonal antibodies (mAbs) against S1 protein of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Methods 6-His-tagged recombinant fragment at N-terminal residues 249 to 667 of SARS-CoV S1 protein including S-protein receptor-binding domain was expressed in E. coli. The immunogenicity of this S1 domain was identified and used to immunize BALB/c mice for the production ofhybridomas. The identification of the mAbs against this S 1 domain was performed using indirect enzyme-linked immunosorbent assay (ELISA),indirect immunofluorescence assay (IFA) and Western blotting, respectively. Results Three hybridomas producing mAbs specific to the S1 domain was obtained, with a relative molecular mass of 48 500. None of the 3 mAbs were reactive with human coronaviruses 229E and OC43. Two of the mAbs were IgG2a isotype, and the other was IgG1. Conclusion This is the first report of mAbs produced against S-protein receptor-binding domain of SARS-CoV. The 3 S1-specific mAbs may be useful for further study of the function of the S protein and for diagnosis of SARS-CoV infection.
Keywords：severe acute respiratory syndrome-associated coronavirusSARS-CoVmonoclonal antibodyspike glycoproteinreceptor-binding domain
Publication Date：2004-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )