Cytoxic T lymphocyte responses to hepatitis B virus small surface antigen in BALB/c mice
Abstract：Objective To observe cytotoxic T lymphocytes (CTL) responses induced by immunization of mice with hepatis B small surface antigen (S-HBsAg). Methods BALB/c(H-2d) mice were injected intraperitoneally with S-HBsAg at the doses ranging from 0-5 μg with a booster injection 2 weeks later. Four weeks after the booster immunization, the specific cytotoxic reactivity of the spleen lymphocytes isolated from the immunized mice, following stimulation by S-HBsAg specific CTL epitope peptide in vitro, with 51Cr-labeled P815 cells treated with the peptide was observed. Results The percentage of specific release of 51Cr in mice immunized with S-HBsAg at the doses of 0, 0.65, 1.25, 2.5, and 5 μg was 31.21%±9.23%, 42.36%±19.32%, 91.21%±22.97%, 69.25%±24.13% and 51.49%±21.661% respectively. In mice receiving the only primary vaccination, the corresponding percentage was 27.34%±14.25%, 32.27%±15.35%, 56.28%±24.35%, 44.34%±18.65% and 40.76±56% respectively. Conclusion Immunization with S-HBsAg efficiently elicits CTL response in BALB/c mice in a dose-dependent manner and enhanced by booster immunization.
Keywords：hepatitis B virussmall surface antigenBALB/c miceimmunizationcytotoxic T lymphocytes
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1242-1244 )