Construction of eukaryotic expression vectors for mouse anti-human fibrin scFv and low- molecular weight single-chain urokinase fusion gene
SHU Dong
XU Bing
YU Wei-yuan
LUO Shen-qiu
Abstract：Objective To construct the eukaryotic expression vector for the fusion gene comprising the genes encoding mouse anti-human cross-linked fibrin single-chain fragment variable (scFv) antibody and low-molecular weight single-chain urokinase. Methods The single peptide of recombinant human ipro-urokinase was ligated with the target fusion gene, and the same reading frame was guaranteed. The ligated compound was subsequently incorporated respectively into pcDNA3 and pMJK expression vectors by DNA recombination technique. Results and Conclusion The desired recombinant plasmids pcDNA3 and pMJK3 containing the signal peptide of recombinant human ipro-urokinase gene and the fusion gene were constructed,which lays the foundation for establishing of a cell line that may secrete the fusion protein.
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Publication Date：2001-04-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 261-262,281 )