Roles of N-methyl-D-aspartate and non-N-methyl-D-aspartate receptors in the respiratory rhythmical discharge of the hypoglossal nerve in the brainstem slice from neonatal rats
PAN Bing-xing
WU Zhong-hai
WANG Ning-Qian
Abstract：Objective To study the role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the generation and modulation of basic respiratory rhythm. Methods Respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve was recorded by suction electrode on the brainstem slices isolated from the neonatal rats, and the effects of the excitatory amino acids and its antagonists on the RRDA were investigated by adding these drugs into the modified Kreb's solution perfusing the brainstem slices. Results After application of the non-NMDA receptors agonist KA, it was found that the respiratory cycle and the expiratory time were slightly lengthened, but the NMDA receptor agonist NMDA had no effect on the RRDA. Both of the mutual antagonist DNQX and AP5 remarkably decreased the discharge frequency and the integral amplitude, accompanied by the shortening of the inspiratory time; DNQA simultaneously shortened the respiratory cycle and the expiratory time. Conclusion During the generation and the modulation of the mammalian respiratory rhythm, NMDA receptors act mainly to regulate the amplitude of the respiratory activity, and the non-NMDA receptors can not only affect the respiratory amplitude but also modulate the respiratory rhythm.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 251-254 )