Studies on purification and renaturation of recombinant human IL-2/GM-CSF fusion protein

LINLAI Xing-mei; ZHOU Ming-qian; CHEN Ze-hong; HU Zhi-ming; LIU Jing; HUANG Shu-qi; WANG Xiao-ning

doi:10.3321/j.issn:1673-4254.2001.04.002

Home>Articles>Studies on purification and renaturation of recombinant human IL-2/GM-CSF fusion protein

DOI: 10.3321/j.issn:1673-4254.2001.04.002

Studies on purification and renaturation of recombinant human IL-2/GM-CSF fusion protein

LINLAI Xing-mei

ZHOU Ming-qian

CHEN Ze-hong

HU Zhi-ming

LIU Jing

HUANG Shu-qi

WANG Xiao-ning

Abstract：Objective To study the purification and renaturation of recombinant human IL-2/GM-CSF fusion protein. Methods After the inclusion bodies were isolated, the optimal conditions of denaturation and renaturation were studied. The renatured recombinant human IL-2/GM-CSF was purified by DEAE-Sepharose FF ion-exchange chromatography. Results The purified product manifested biological activity with purity of up to 95%. Conclusions The procedures employed in this study is simple and reliable.

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Publication Date：2001-01-01

Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)

Pages：3( 245-247 )

Journal of Southern Medical University

PKUISTIC

ISSN：1673-4254

Year, Vol.(Issue)：2001,21(4)