Establishment of a canine model of chronic multicoronary stenosis

doi:10.3321/j.issn:1673-4254.2001.01.020

Abstract：Objective To establish a large animal model of chronicmulticoronary stenosis or occlusion that mimics human condition of coronary artery disease(CAD). Methods Nineteen adult dogs were used to establish chronic multicoronary stenosis or/and occlusion by installation of Ameroid constrictors at the proximal portion of the left anterior descending (LAD) and the left circumflex coronary arteries(LCX). The wall thickening percentage(%WT)was measured with 2D-echocardiography (2DE) and myocardial blood flow (MBF) with radiolabeled microspheres. Baseline 2DE was performed immediately prior to surgery. At postoperative days 2, 7, 14, 21, 28 and 42,2DE was performed and radiolabled microspheres administered. Results Thirteen dogs completed the whole experiment. At day 7, regional wall motion abnormality (RWMA) was presented in 26 myocardial sections . The average %WT significantly decreased in both LAD and LCX at day 7, while the decrease in MBF was not observed until day 21. At day 42, RWMA was seen in 36 out of 50 sections. The average %WT in both LAD and LCX beds was still lower than the baseline. MBF in the LCX bed decreased further, which returned to nearly the baseline levels. Conclusion This canine model of chronic mutilcoronary stenosis simulating the human condition of CAD produces significant RWMA and is appropriate for the study of chronic reversible myocardial contractile dysfunction.