Excitability of cocultured cerebral cortex neurons and astrocytes to tert-butyl hydroperoxide cytotoxicity
Abstract：Objective To investigate the response of neurons and astrocytesfrom Sprague Dawley rat brain against tert-buty1 hydroperoxide(tbOOH) toxicity,both separately and in cooperation, for the purpose of exploring the interrelation of the two kinds of cells. Methods Cell viability of singly cultured neurons or astrocytes from the cerebral cortex was compared by MTT reduction assay,and the morphological changes of cocultured neurons and astrocytes were observed upon tbOOH attack. Results The difference of cell viability was insignificant(P>0.05) between the neurons and astrocytes, but the damage of the neurons exceeded that of the astrocyte in terms of severity in mixed culture. Conclusion The astrocytes is very likely to possess better ability to fight tbOOH toxicity than the neurons do when they are cultured together, suggesting that the protection of neuron against active oxygen species toxicity is actually executed by the astrocyte.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 32-33,36 )