Overproduction of manganese superoxide dismutase modulates H2O2-induced apoptosis in transfected human PC12 cells
ZHANG Bao
MO Yong-yan
ZHOU Mei
CHEN Yuan
Abstract：Objective To explore the effect and mechanism of manganese superoxide dismutase (MnSOD): on H2O2-induced apoptosis of PC12 cells. Methods PC12 cells were transfected with sense and antisense human MnSOD by lipofectin, and exposed to 50 mmol/L H2O2, MTT assay, low cytometry and Hoechst stain were used to study the cytotoxicity. Results MnSOD activity increased by 130% in cells transfected with sense MnSOD cDNA, and was decreased by 67% in cells transfected with antisense MnSOD cDNA as compared with controls. Conclusion Antisense MnSOD can offer protection against H2O2-induced apoptosis in PC12 cells. The damage of the mitochondria may be attributed to the cytotoxicity of H2O2 against PC12 cells, which induces imbalance of cellular redox and apoptosis.
Keywords：MnSODgene transfectionPC12 cellsH2O2apoptosis
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 510-512 )