DOI: 10.1002/ame2.70072
Paternity testing for crab-eating macaques and rhesus macaques using microsatellite markers
Yiming Yuan
Tianqi Sun
Lu Zhang
Wensheng Zhang
Teng Meng
Jianhua Zheng
Rui Zhang
Lei Lu
Zirong Pu
Yan Li
Yefeng Qiu
Abstract：Accurate macaque paternity identification is of great significance in various fields, yet relevant research remains scarce. Our study aimed to screen effective microsatellite markers for macaque paternity testing. Initially, 300 microsatellite markers were randomly selected from the genome of the crab-eating macaque (Macaca fascicularis), and 12 highly polymorphic tetra-nucleotide repeat markers were identified. These markers' genetic parameters and exclusion probabilities in both crab-eating and rhesus macaque (Macaca mulatta) populations were calculated, meeting the paternity testing requirements for both species. To validate the markers, 16 crab-eating macaque and 10 rhesus macaque families with known pedigrees were randomly chosen for testing. The genotypes of the 12 markers in the macaques' offspring could be traced back to their parents, confirming the accuracy and applicability of the marker combination for paternity identification in both macaque species.
Keywords：crab-eating macaquesmicrosatellitepaternity testrhesus macaques
Publication Date：2025-09-30
Online Publishing Date：2025-10-28(First online date of this platform, not the publication date of the document)
Pages：7( 1710-1716 )