DOI: 10.1002/ame2.70019
Dual-energy X-ray absorptiometry for detecting neurogenic pulmonary edema in a mouse model of subarachnoid hemorrhage
Tatsushi Mutoh
Hiroaki Aono
Yushi Mutoh
Tatsuya Ishikawa
Abstract：Murine subarachnoid hemorrhage (SAH) induced using the filament perforation method is a useful in vivo experimental model to investigate the pathophysiological mechanisms in the brain underlying SAH. However, identifying mice with comorbid acute neurogenic pulmonary edema (NPE), a life-threatening systemic consequence often induced by SAH, in this model is difficult without histopathological investigations. Herein, we present an imaging procedure involving dual-energy X-ray absorptiometry (DXA) to identify NPE in a murine model of SAH. We quantified the lung lean mass (LM) and compared the relationship between micro-computed tomography (CT) evidence of Hounsfield unit (HU) values and histopathological findings of PE. Of the 85 mice with successful induction of SAH by filament perforation, 16 (19%) had NPE, as verified by postmortem histology. The DXA-LM values correlate well with CT-HU levels (r=0.63, p＜0.0001). Regarding the relationship between LM and HU in mice with post-SAH NPE, the LM was positively associated with HU values (r2=0.43; p=0.0056). A receiver operating characteristics curve of LM revealed a sensitivity of 87% and specificity of 57% for detecting PE, with a similar area under the curve as the HU (0.79±0.06 vs. 0.84±0.07; p=0.21). These data suggest that confirming acute NPE using DXA-LM is a valuable method for selecting a clinically relevant murine NPE model that could be used in future experimental SAH studies.
Keywords：dual-energy X-ray absorptiometrymouse modelneurogenic pulmonary edemasubarachnoid hemorrhage
Publication Date：2025-06-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1146-1151 )