DOI: 10.1002/ame2.12474
Processing-induced reduction in dianthrones content and toxicity of Polygonum multiflorum:Insights from ultra-high performance liquid chromatography triple quadrupole mass spectrometry analysis and toxicological assessment
Wan-Fang Li
Ying Wang
Cai-Xia Qiu
Jie Li
Jie Bao
Jian-Bo Yang
Hong-Tao Jin
Abstract：Background: Polygonum multiflorum-induced liver injury (PM-DILI) has significantly hindered its clinical application and development.
Methods: This study investigates the variation in content and toxicity of dianthrones, the toxic components of P. multiflorum, during different processing cycles. We employed the ultra-high-performance liquid chromatography triple quadrupole mass spectrometry method to quantify six dianthrones in raw P. multiflorum and formulations processed with a method called nine cycles of steaming and sunning. Additionally, toxicity assessments were conducted using human normal liver cell line L02 and zebrafish embryos.
Results: Results indicate a gradual reduction in dianthrones content with increasing processing cycles. Processed formulations exhibited significantly reduced cytotoxicity in L02 cells and hepatotoxicity in zebrafish embryos.
Conclusions: Our findings elucidate the relationship between processing cycles and P. multiflorum toxicity, providing theoretical support for its safe use.
Keywords：dianthroneshepatotoxicityPolygonum multiflorum Thunb.ultra-high performance liquid chromatography triple quadrupole mass spectrometry(UHPLC-QQQ-MS/MS)zebrafish embryos
Publication Date：2025-04-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 685-695 )