DOI: 10.1002/ame2.12392
Conditional knockout of the NSD2 gene in mouse intestinal epithelial cells inhibits colorectal cancer progression
Mengyuan Li
Hanxue Chen
Xingjiu Yang
Wenlong Zhang
Chengyan Ma
Qinghong Wang
Xinpei Wang
Ran Gao
Abstract：Background: Nuclear receptor-binding SET domain 2 (NSD2) is a histone methyltransferase that catalyzes dimethylation of lysine 36 of histone 3 (H3K36me2) and is associated with active transcription of a series of genes. NSD2 is overexpressed in multiple types of solid human tumors and has been proven to be related to unfavorable prognosis in several types of tumors.
Methods: We established a mouse model in which the NSD2 gene was conditionally knocked out in intestinal epithelial cells. We used azoxymethane and dextran sodium sulfate to chemically induce murine colorectal cancer. The development of colorectal tumors were investigated using post-necropsy quantification, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA).
Results: Compared with wild-type (WT) control mice, NSD2 fl/fl-Vil1-Cre mice exhibited significantly decreased tumor numbers, histopathological changes, and cytokine expression in colorectal tumors.
Conclusions: Conditional knockout of NSD2 in intestinal epithelial cells significantly inhibits colorectal cancer progression.
Keywords：colorectal cancerNSD2 fl/fl-Vil1-Cre micenuclear receptor-binding SET domain 2
Publication Date：2025-02-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 322-331 )