DOI: 10.1002/ame2.12385
Dyssynchronous heart failure models in canines:New insights into electrocardiographic,echocardiographic and histological features
Han Jin
Shengwen Yang
Hao Huang
Sijing Cheng
Pengkang He
Sixian Weng
Min Gu
Hongxia Niu
Wei Hua
Yiran Hu
Hui Li
Abstract：Background: We investigated the similarities and differences between two experimental approaches using tachy-pacing technology to induce desynchronized heart failure in canines.
Methods: A total of eight dogs were included in the experiment, four were tachy-paced in right ventricle apex (RVAP) and 4 were paced in right atrium after the ablation of left bundle branch to achieve left bundle branch block (RAP+LBBB). Three weeks of follow-up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment.
Results: Both experimental approaches successfully established heart failure with reduced ejection fraction models, with similar trends in declining cardiac function. The RAP+LBBB group exhibited a prolonged overall ventricular activation time, delayed left ventricular activation, and lesser impact on the right ventricle. The RVAP approach led to a reduction in overall right ventricular compliance and right ventricular enlargement. The RAP+LBBB group exhibited significant reductions in left heart compliance (LVGLS, %: RAP+LBBB -12.60±0.12 to -5.93±1.25; RVAP -13.28±0.62 to -8.05±0.63, p=0.023; LASct, %: RAP+LBBB -15.75±6.85 to -1.50±1.00; RVAP -15.75±2.87 to -10.05±6.16, p=0.035). Histological examination revealed more pronounced fibrosis in the left ventricular wall and left atrium in the RAP+LBBB group while the RVAP group showed more prominent fibrosis in the right ventricular myocardium.
Conclusion: Both approaches establish HFrEF models with comparable trends. The RVAP group shows impaired right ventricular function, while the RAP+LBBB group exhibits more severe decreased compliance and fibrosis in left ventricle.
Keywords：dyssynchronyheart failuretachy-pacing technology
Publication Date：2025-01-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：12( 142-153 )