Pathological targets and their mechanisms in post-stroke dementia

Zhou Yanxia; Shen Jinglin

doi:10.26939/j.cnki.CN11-9353/R.2025.02.003

Abstract：Objective To explore the pathological targets and their mechanisms in post-stroke dementia(PSD)based on GEO database and bioinformatics.Methods The mRNA gene chip dataset GSE186798,including neuronal cells,astrocytes and endothelial cells from normal patients,PSD patients and post-stroke non-dementia(PSND)patients,was downloaded from the GEO database.Differential expression genes were screened using the criteria of|log Fold Change|>1 and P value<0.05.The differential expression genes were subjected to GO and KEGG enrichment analysis using the DAVID database.The protein-protein interaction(PPI)network was constructed using Cytoscape software,and core genes were screened.Core gene-related miRNAs were predicted using TargetScanHuman,miRTraBase,and miRWalk databases,and an mRNA-miRNA network was constructed.Results There were 468 differential expression genes in neuronal cells between PSND patients and PSD patients,including 306 up-regulated genes and 162 down-regulated genes.GO enrichment analysis showed that PSD was mainly related to such biological functions as neuronal migration,protein binding,nucleus,and enzyme activity.KEGG enrichment analysis indicated that PSD was closely associated with TNF signaling pathway and HIF-1 signaling pathway.Ten core genes were screened from the PPI network,including EP 300,H 2 AC 20,H 2 BC 14,H2 BC5,AGO4,RBBP5,H2 AC13,UIMC1,KMT5 A and BAZ2 A.Has-miR-124 was a common miRNA for the ten core genes.Conclusion The ten core mRNA targets and has-miR-124 miRNA may be the main lesion targets of PSD,and TNF and HIF-1 signaling pathways may be part of the mechanisms to treat PSD.This study reveals the pathogenesis of PSD,providing new directions for the clinical treatment of PSD.