Preliminary study on the inhibitory effect of platycodin d on the growth and migration of hypertrophic scar fibroblasts
KONG Xu
ZHAO Hai
HUI Qiang
ZHOU Dapeng
HUANG Yue
XU Liang
ZHANG Yuancheng
Abstract：Objective To investigate the effects of platycodin D on the proliferation,apoptosis,migration,and AKT activity of hypertrophic scar fibroblasts.Methods Hypertrophic scar fibroblasts were treated with 0 μ mol/L,20 μmol/L,40 μmol/L,or 80 μmol/L platycodin D.Cell proliferation was assessed via MTT assay.Cell cycle distribution was analyzed by PI staining after 24-hour treatment,and apoptosis was detected using Annexin V-PI staining.Cell migration was evaluated using the Transwell assay.Western blotting was performed to examine the expression of Cyclin D1,Bcl-2,MMP-2,phospho-AKT(P-AKT),and total AKT proteins.For further analysis,cells were divided into four groups:control(0 μmol/L),platycodin D(40 μmol/L),LY294002 alone(10 μmol/L),and platycodin D(40 μmol/L)+LY294002(10 μmol/L).Cell proliferation,migration,and P-AKT activation were assessed by MTT,Transwell assays,and western blotting.Result The MTT assay results indicated that platycodon-D at different concentrations significantly inhibited cell proliferation,and the inhibitory effect became more pronounced with increasing drug concentration and treatment duration.Cell cycle analysis showed that platycodon-D at different concentrations upregulated the proportion of cells in the G1 phase and downregulated the proportion of cells in the S phase.Annexin V-PI staining results indicated that platycodon-D at different concentrations significantly promoted apoptosis.The transwell assay results showed that platycodon-D at different concentrations significantly inhibited cell migration.Western blot demonstrated that platycodon-D downregulated the expression of Cyclin D1,Bcl-2,MMP2,and P-AKT proteins.Pretreatment with LY294002 did not further enhance the inhibitory effects of platycodon-D on proliferation,migration,and P-AKT activation.Conclusion Platycodin D can inhibit the activation of P-AKT and downregulate the protein expression of Cyclin D1,Bcl-2,and MMP2,thereby suppressing the growth and migration of hypertrophic scar fibroblasts.
Keywords：platycodin Dhypertrophic scarfibroblastsgrowth
Publication Date：2025-11-25
Online Publishing Date：2025-12-25(First online date of this platform, not the publication date of the document)
Pages：6( 419-424 )