Proliferative effect of platelet-rich plasma derived from human umbilical cord blood with different concentrations on hu-man umbilical cord-derived mesenchymal stem cells
YAN li
ZHAO Pan-pan
WANG Xi-liang
YU Yue-xin
YU Jia-ping
ZHANG Jing-rui
Abstract：Objective Explore the proliferative effect of platelet-rich plasma(PRP)from human umbilical cord blood at different concentrations on human umbilical cord-mesenc hymal stem cells(hUC-MSCs)and the optimal concentration for proliferation.Methods Umbilical cords of full-term healthy pregnant and lying-in women were collected.hUC-MSCs were isolated and cultured by the tissue block culture method.Collect umbilical cord blood.Isolate PRP by the two-step centrifugation method and activate it.The concentration of transforming growth factor-β1 in platelet-rich plasma is measured by enzyme-linked immunosorbent assay,which represents the PRP concentration.According to the PRP concentration,the samples are divided into 7 groups:0 pg/ml group,500 pg/ml group,1 000 pg/ml group,1 500 pg/ml group,2 000 pg/ml group,2 500 pg/ml group,and 3 000 pg/ml group.Inoculate hUC-MSCs,Measure the absorbance using thecell counting kit-8 kit every 24 hours.Statistically analyze the proliferative effect of PRP at different concentrations on hUC-MSCs and the optimal concentration for proliferation.Results On the 4th day of culture,the absorbance values in the 1 000 pg/ml and 1 500 pg/ml groups were higher than those in the 0 pg/ml group,the differences were statistically significant(P<0.05).The absorbance value in the 3 000 pg/ml group was lower than that in the 0 pg/ml group,the difference was statistically significant(P<0.05).On the 5th,6th,and 7th days,the absorbance values in the 500 pg/ml,1 000 pg/ml,1 500 pg/ml,and 2 000 pg/ml groups were all higher than those in the 0 pg/ml group.Among these,the 1500 pg/ml group exhibited the highest absorbance value,the differences were statistically significant(P<0.05).The absorbance values in the 2 500 pg/ml and 3 000 pg/ml groups were lower than those in the 0 pg/ml group,the differences were statistically significant(P<0.05).Conclusion PRP derived from source of human umbilical cord blood promotes the proliferation of hUC-MSCs,the optimal concentration to promote the proliferation of hUC-MSCs was 1 500 pg/ml.
Keywords：Platelet-rich plasmaHuman umbilical cord-mes enchymal stem cellsCell proliferation
Publication Date：2025-09-25
Online Publishing Date：2025-11-06(First online date of this platform, not the publication date of the document)
Pages：6( 376-381 )