Establishment of an acute kidney injury animal model in rats by deep hypothermic circulatory arrest
WANG Huan
GAO Yan
LIANG Bing
SHI Yun
LIU Yu
Abstract：Objective To establish an animal model of acute kidney injury(AKI)by deep hypothermic circulatory arrest(DHCA)in rats.Methods A total of 18 adult male SPF-grade Sprague-Dawley rats were selected,aged(12.6±0.5)weeks old,(ranging from 12 to 13 weeks old),with a body weight of(376.9±13.6)g,ranging from 350 to 400 g.The rats were randomly divided into sham operation group(n=6),cardiopulmonary bypass(CPB)group(n=6),and CPB+DHCA group(n=6)by a random number table.The sham operation group underwent only anesthesia induction,tracheal intubation,and femoral artery/vein and internal jugular vein catheterization,and rectal temperature was maintained above 35℃.The CPB group underwent full-flow perfusion for 90 minutes while rectal temperature was maintained 35℃to 36℃.In the CPB+DHCA group,after initiating CPB,the rats'rectal temperature was lowered below 25℃to induce cardiac arrest.After 30 minutes of circulatory arrest,circulation was restored with gradual flow increase and slow rewarming to 35℃to 36℃,maintaining a total CPB duration of 90 minutes.Postoperatively,the rats surviving beyond 24 hours were euthanized,and arterial blood was collected aseptically via the abdominal aorta to measure creatinine and urea nitrogen levels.Bilateral renal tissues were harvested for sectioning.HE staining was used to observe the tissue morphology and Tunel staining was used to detect the apoptotic cells.Results All the rats in the sham operation and CPB groups survived for 24 hours postoperatively,while one rat in the CPB+DHCA group died within 24 hours.There was no statistically significant difference in the levels of creatinine and urea nitrogen between the sham operation group and the CPB group(P>0.05).The levels of creatinine and urea nitrogen in the CPB+DHCA group were higher than those in the sham operation group and the CPB group,and the differences were statistically significant(P<0.05).The apoptotic index of renal tubular epithelial cells in the CPB+DHCA group was higher than that in the sham operation group and the CPB group,and the difference was statistically significant(P<0.05).Conclusion A standardized 30-minute DHCA protocol successfully establishes a rat AKI model post-DHCA,providing blood and tissue samples for subsequent mechanistic and interventional studies.
Keywords：acute kidney injurydeep hypothermic circulatory arrestcardiopulmonary bypassanimal model
Publication Date：2025-09-25
Online Publishing Date：2025-11-06(First online date of this platform, not the publication date of the document)
Pages：5( 346-349,365 )