Study on the mechanism of nuclear factor E2 related factor 2 and heme oxygenase-1 in liver injury of exertion-induced heat stroke rats
WANG Ling
WANG Lin-lin
GUO Jun-feng
JIN Hong-xu
ZHANG Fu-yuan
CHEN Xiang
GAO Yan
Abstract：Objective To observe the pathological changes of liver injury and the expression of Nrf2/HO-1 in exertional heat stroke rats.Methods Healthy male SD rats were randomly divided into blank group(Con group)and exertional hyperthermia group(EHS 2 h group,EHS 6 h group and EHS 12 h group)with 6 rats in each group.The experiment was started after all rats underwent gradient intensity adaptive running.Con group rats were fed normally after adaptive treadmill training.The exertional heat stroke group was given treadmill training until the rats developed exertional heat stroke.EHS group rats were randomly selected 2 h,6 h and 12 h after the onset of the disease,and liver tissue was reserved for use.The liver morphology was observed by HE staining.The expression of Nrf2,pNrf2 and HO-1 proteins in the liver of rats was detected by immunohistochemical staining.Results Compared with the normal group,the hepatocyte swelling,cytoplasmic steatosis and hepatocyte necrosis were obvious,and the portal vein was congestion and dilatation.Immunohistochemical staining showed that the expression of Nrf2,pNrf2 and HO-1 in positive cells in EHS group was significantly different from that in normal group at different time points(P<0.05).Conclusion Nrf2/HO-1 signaling pathway has protective effect on liver injury in exertional heat stroke rats.
Keywords：Exertional heat strokeLiver injuryNuclearfactor-erythroid 2-relatedfactor 2Phosphorylation nuclearfactor-ery-throid 2-relatedfactor 2Heme oxygenase-1
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 301-304 )