Protective effect and mechanism of proanthocyanidins on acute lung injury induced by hypothermia in mice

CAO Bin-yan; DAI Jing; YOU Ping-fei; HU An; YU Xin-hui; JIN Hong-xu; LIU Ying

doi:10.16048/j.issn.2095-5561.2024.05.03

Abstract：Objective Explore the protective effect and mechanism of proanthocyanidins on acute lung injury in mice caused by severe accidental hypothermia.Methods 45 mice were randomly divided into three groups of 15 mice each,namely,the CON-operated group(CON group),the model group(CE group),and the intervention group(CE+PCs group)group.The CoN group and CE group was administered by gavage 10 mg/kg distilled water for 7 consecutive da The CE+PCs group was administered by gavage 500 mg/kg proanthocyanidin solution once daily for 7 consecutive days.CE group and CE+PCs group were killed on the 8th day after establishing lung injury model caused by severe accidental hypothermia.The general changes of lung tissue in each group were observed.Lung histopathological changes were observed and lung injury score was performed.The wet dry ratio of pulmonary was calculated by weighing them.ELISA was used to detect the concentrations of TNF-α and IL-6 in lung tissue homogenates.Additionally,Western blot was used to measure the expression levels of TLR4,NFκB-P65 and IκBα in lung tissues.Results The lung injury score of CE group was higher than that of CON group.The lung injury score in CE+PCs group was lower than that in CE group.The lung wet dry ratio in CE group was lower than that in CON group.The lung wet dry ratio in CE+PCs group was lower than that in CE group.The lung injury score of CE+PCs group was lower than that of CE group.The concentrations of TNF-α and IL-6 in lung homogenate of mice in CE group were higher than those in CON group.The concentrations of TNF-α and IL-6 in lung homogenate of mice in CE+PCs group were lower than those in CE group.The expression of TLR4 and NFκB-P65 in lung tissue of CE group was higher than that of CON group,and the expression of IκBα was lower than that of CON group.The expression of TLR4 and NFκB-P65 in lung tissues of CE+PCs group was lower than that of CE group,and the expression level of IκBα was higher than that of CE group,the differences were statistically significant(P<0.05).Conclusion Severe accidental hypothermia can lead to acute lung injury in mice,a process that may be associated with activation of inflammatory pathways.Proanthocyanidins protect against severe accidental hypothermia-induced acute lung injury by inhibiting the TLR4/NFκB pathway and reducing the inflammatory response.