Organ protection of ischemic postconditioning during deep hypothermic circulatory arrest

ZHANG Ren-teng; LIU Yu; LI Ning; XU Shu; TANG Liang; ZHANG Yong

doi:10.16048/j.issn.2095-5561.2019.01.01

Abstract：Objective To investigate the effect of ischemic postconditioning (IPOC) on organ protection after deep hypothermia circulatory arrest (DHCA). Methods A total of 12 healthy adult Bama minipigs were randomly divided into the Group A (DHCA group) and Group B (DHCA+IPOC group), with 6 pigs in each group.Two groups were established by the common carotid artery and internal jugular vein intubation extracorporeal circulation, transfer flow and cooling to the rectal temperature of 15℃, the suspension loop 90 minutes after recovery of extracorporeal circulation and temperature, successively from the extracorporeal circulation, then breathing machine auxiliary.At the beginning of the recovery of extracorporeal circulation, Group B carried out perfusion of 30 seconds/block of30 seconds for 3 cycles.The alanine transaminase (ALT), glutamic oxalacetic transaminase (AST), creatinine (Cr) and troponin (TNT) of the two groups of experimental animals were measured with blood drawn 1, 3 and 7 days after operation.Results All animals passed through perioperative period successfully.There was no statistically significant difference (P＞0.05) in the vital signs of blood pressure, heart rate and rectal temperature of the two groups at the time points of anesthesia induction, initial transposition, cessation of circulation, cardiac repulse, and extracorporeal circulation.On the first day after surgery, ALT, Cr and TNT were significantly higher in Group A than those of Group B (P＜0.05). The AST of the two groups showed no significant difference (P＞0.05). On the3rd and 7th day after surgery, the above indicators were still slightly higher in Group A than those of Group B, while the difference was not statistically significant (P＞0.05). Conclusion IPOC can help to alleviate multiple organ injury of liver, kidney and heart after DHCA.The benefit in the first day after operation is more significant.