Effects of JAK/STAT3 inhibitor on rabbit traumatic shock renal injury
LIU Xue-lei
LIU Yun-en
TONG Zhou
TONG Chang-ci
ZHANG Yu-biao
SHI Lin
CONG Pei-fang
SHI Xiu-yun
LIU Ying
JIN Hong-xu
HOU Ming-xiao
Abstract：Objective To explore the effect of JAK / STAT3 signaling pathway inhibitor AG490 in traumatic shock induced kidney damage. Methods A total of 30 New Zealand rabbits were divided into control group, model group and AG490 treatment group,with 10 rats in each group. Rabbits were made traumatic shock and kidney damage in model group and AG490 treatment group. To detect the expression of STAT3,Kim-1 and TGF-β1 in kidney tissues via real-time quantitative PCR and western blot. Results Compared with control group,renal creatinine and urea nitrogen levels in model group were significantly higher than those in the control group(P<0. 05);compared with the model group,serum creatinine and blood urea nitrogen levels in AG490 treatment group were significantly lower(P<0. 05);compared with the control group,levels of expression of STAT3 and TGF-β1 mRNA protein in kidney tissue in the model group were significantly increased ( P<0. 05);compared with the model group,STAT3 and TGF-β1 mRNA and protein expression level in AG490 treatment group were significantly reduced(P<0. 05). Conclusion AG490 may be blocked by TGF-β1 activated in JAK/STAT3 signaling pathway,thereby inhibiting the development of traumatic shock and kidney damage.
Keywords：Traumatic shockKidney injuryJAK/STAT3 signaling pathwayTGF-β1AG490
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 18-21 )