Effects of β2-adrenergic receptor on expressions of PGC-1 in rat model of hemor-rhagic shock
ZHANG Cheng
GAO Guang-rong
LI Da
SHAN Yong-qi
ZHANG Xue-feng
Abstract：Objective To investigate the effects of β2-adrenergic receptor on expressions of PGC-1 in skeletal muscles in rat model of hemorrhagic shock.Methods ds SD rats were randomized into three groups:Control(C),Hemorrhage(H)and Hemorrhage+AR-β2(N).Venous blood(22.5 ml/kg)was continously withdrawn over 10 minutes to establish hemorrhagic shock model.Selective β2-adrenoceptor antagonist ICI 118,551 (0.5 mg/kg) was intravenously administered after hemor-rhage had finished.After 30 minutes shock periods,rats received whole blood and lactated Ringer′s solution resuscitation (1:2)for 30 minutes.Rats were monitored for 8h after resuscitation.Hematocrit(Hct),Base excess(BE),Lactate(Lac)and Glucose(Glu)were recorded at the baseline and different hemorrhagic shock time (HST).The expressions of PGC-1 mRNA and protein in skeletal muscles were tested by real-time reverse transcription polymerase chain reaction ( qRT-PCR ) and western blotting .Results Rats in Group N had lower lactate levels and higher BE levels than Group H ( P<0.05 ) .At 72 hours,there were five survivors in Group H and eight in Group N (P<0.05 versus Group H,Log Rank).Both Group H and Group N increased PGC-1 mRNA and protein expressions at 2 hours after resuscitation,but more higher expressions were seen in Group H compared with Group N (P<0.05).Thereafter,the expressions of PGC-1 mRNA and protein decreased gradually in both groups ,and no statistic differences were found between the two groups .Conclusion Adrenergic receptor-β2 could regulate energy metabolism balance through adaptive regulation to PGC-1 during hemorrhagic shock .
Keywords：Hemorrhagic shockβ2-adrenergic receptorPeroxisome proliferator activated receptor gamma coactivator-1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 220-224 )