Effect of extracorporeal membrane oxygenation on the expression of cytokines in a-cute lung injury induced by impact injury
TONG Chang-ci
LIU Yun-en
ZHANG Yu-biao
SHI Lin
LIU Ying
LIU Xue-lie
CONG Pei-fang
JIN Hong-xu
GAO Yan
HOU Ming-xiao
Abstract：Objective To investigate the specific cytokines in the acute lung injury caused by severe trauma with the treatment of extracorporeal membrane oxygenation(ECMO). Methods Twenty beagle dogs were randomly divided into 4 groups:normal control group,model group,mechanical ventilation treatment group,and ECMO treatment group. Rubber bul-lets hit to the canine model of lung injury. To extract total protein of the lung tissue,proteinchip assayed and screened differ-entially expressed cytokines. Results In the detection of the 20 cytokines,compared with the control group,IL-2,IL-6,IL-8,IL-10,MCP-1,SCF,HGF and HGF R,IL-1B,MIP-1βand TNF RI B of model group,expressed increasingly,which de-creased after mechanical ventilation and ECMO treatment,and the difference of ECMO treatment was more obvious. The ex-pression tendency of VEGF,FGF-7 and IL-12p40 were opposite. The expression of GM-CSF,TNF alpha and IL-17A had no significant difference. EPO and IFNγhad no expression. Conclusion ECMO treatment can improve the inflammatory re-sponse caused by severe trauma induced acute lung injury,and provide direction and target for the subsequent study of mo-lecular mechanism of the acute lung injury caused by severe trauma with the treatment of ECMO.
Keywords：Acute lung injuryExtracorporeal membrane oxygenationMechanical ventilationProtein chip
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 6-9 )