Effect of ZnSO4 Precondition on Myocardium Celluar Cx43 Protein during Ischemia/Reperfusion and Reperfusion Ventricular Arrhythmias in Rats
MA He
LI Ruofan
ZHANG Zhao
YAO Tianming
Abstract：Objective To investigate the arrhythmia and the phosphorylation of gap junction protein Connexin43 (Cx43 ) during the ischemia/reperfusion injury in rat heart and effects of ZnSO4 (zinc)precondition on it.Methods The male SD rats were randomly divided into 4 groups:sham with saline precondition group(Sham+Sa),sham with ZnSO4 precondition group(Sham+Zn),ischemia/reperfusion with saline precondition group(I/R+Sa),ischemia/reperfusion with ZnSO4 pre-condition group(I/R+Zn)(n=6 in each).The incidence of ventricular arrhythmias and arrhythmia score were observed and the phosphorylation level of Cx43 was evaluated by Western-Blot in each group.Results Compared with the Sham+Sa group and Sham+Zn group,ventricular arrhythmia score in I/R+sa group was obviously increased (P<0.01 ),and the phosphorylation level of Cx43 in ventricular myocardial cells was also significantly increased (P<0.01 ).Compared with the I/R+Sa group,ventricular arrhythmia score in I/R+Zn group obviously decreased (P<0.01 ),and the phosphoryla-tion level of Cx43 in ventricular myocardial cells was also significantly decreased (P<0.01).Conclusion ZnSO4 precon-dition can effectively reduce the incidence of reperfusion ventricular arrhythmias which may be associated with the modula-tion of Cx43 phosphorylation during ischemia/reperfusion in rats.
Keywords：ischemia/reperfusionZnSO4 preconditionConnexin43ventricular arrhythmia
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 97-100 )