Test and Selection of Effect of Silencing AQP4 Gene with Chemical Synthesis siRNA
SONG Zhenquan
ZHAO Xu
LIU Enzhi
LIU Yun-en
ZHANG Xing
ZHANG Haisong
SHAN Tikun
ZHAO Hang
Abstract：Objective Prepared several small interfering RNAs (siRNA)for AQP4 protein in rat brain by chemical synthe-sis.The silencing effect of two chains were verified and selected the one which was more effective.Methods Select two dif-ferent target sequences of the rat according to the AQP4 mRNA.Two siRNAs were synthesised by chemical synthesis,mean-while negative siRNA was synthesised as control.18 male adult Wistar rats were randomly divided into negative siRNA in-jection group,725 line injection group and 1006 line injection group.Corresponding drugs were injected into the left ventri-cle respectively.All animals were executed after 6 hours.The protein expression of AQP4 was detected by immunohisto-chemical staining and the AQP4mRNA expression was detected by in situ hybridization.Results 6 hours after injection,the expression of AQP4 protein and AQP4 mRNA of brain in the 725 line injection group were significantly lower than those of the 1006 line injection group and the negative siRNA injection group (P<0.05).Conclusion 725 line injection could ef-fectively silence the expression of AQP4 in rat brain in 6 hours.
Keywords：RNA interferenceaquaporin4chemical synthesissilencing effect
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 73-76 )