Protective effect of erythropoietin on the early post-traumatic epilepsy rats and its effects on the expression of bcl-2 and caspase-10

WANG Wen; QIAO Wei; DU Chen; HUANG Qi-jun; SHI Quan-hong; XIE Yan-feng; FENG Ai-ping

doi:10.3969/j.issn.1009-4237.2018.05.013

Abstract：Objective To investigate the effects of erythropoietin on the expression of B cell lymphoma-2 (bcl-2)and cysteinylaspartate specific proteinase-10(caspase-10)in cortical brain tissue,and to explore the pro-tective effect on the early post-traumatic epilepsy rats.Methods Thirty adult rats were randomly divided into 3 groups.Rats in the control group were injected with 5 μL normal saline only in the left sensori-motor cortex;rats in the model group and experimental group were injected with the same amount of 100 mmol/L FeCl 3 at the same posi-tion.After 30 min,the rats in the experimental group were injected with rHuEPO 5000 U/kg.The rats in the model group were injected with equal volume of saline.Changes in behavior were observed.After 24 hours,the mRNA and protein expression of bcl-2 and caspase-10 in rat cortical brain tissue were detected by real-time fluorescence quanti-tative PCR and Western blotting.Results (1)Behavior:there was no epileptic seizure in the control group.The seizure frequency and attack time in the experimental group was lower than those in the model group(P<0.05). (2)RT-PCR:bcl-2 mRNA expression in the model group and the experimental group was lower than in the control group,which was higher in the experimental group than in the model group(P<0.05);caspase-10 mRNA expres-sion in the model group and the experimental group was higher than the control group,which was lower in the experi-mental group than the model group(P<0.05).(3)Western blotting detection:the protein expression of bcl-2 in the model group and the experimental group was lower than that in the control group(P<0.05).The expression of bcl-2 protein in the experimental group was higher than that in the model group(P<0.05).The protein expression of caspase-10 in the model group and the experimental group was higher than that in the control group.The expres-sion of caspase-10 protein in the experimental group was lower than that in the model group(P<0.05).Conclu-sion The expression of bcl-2 is decreased and the expression of caspase-10 is increased in the cortical brain tissues of epileptic rats.EPO has a certain protective effect on the brain of iron-induced epilepsy rats through increasing the expression level of bcl-2 and reducing the expression level of caspase-10 in the cerebral cortex and reducing the fre-quency and degree of epileptic seizure.