Effect of selective decontamination of the digestive tract on MDAconcentration and SOD,GSH-Px activities of small intestinal tissuein rabbits with complete paraplegia
NIE Hai
LIU Peng
CAO De-qian
BAI Chun-hong
ZHOU Ji
LIU Qi-dong
AN Hong
Abstract：Objective To explore effects of selective digestive decontamination(SDD)on the content of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and its effects on oxi-dative stress reaction in small intestinal mucosa in paraplegic rabbits.Methods Forty rabbits were subjected to a complete paraplegia following spinal cord injury,and divided into SDD-treated(n=20)and control groups(n=20). The treatment group received SDD using PTA(polymyxin,tobramycin and amphotericin B)protocol by gavage three times daily for 4d after injury.The control group was given the same amount of normal saline.Samples from the small intestine were collected to detect MDA content,SOD and GSH-Px activities and to assess morphological chan-ges in the small intestine,using Hematoxylin and eosin staining.The correlation between MDA,SOD and GSH-Px levels and the degree of intestinal mucosal damage were analysed.Results The content of MDA[(3.58 ±0.98) nmol/mgprot vs.(8.19 ±1.22)nmoL/mgprot,P<0.001]in intestinal tissue was significantly lower in the SDD group than those in the control group(P<0.001).The activities of SOD[(57.2 ±9.4)U/mgport vs.(42.7 ± 13.4)U/mgport]and GSH-Px[(111.6 ±12.0)U/mgport vs.(89.7 ±11.0)U/mgport]were obviously higher in the SDD group than those in the control group.Correlation alalysis showed a positive correlation between MDA con-tent and degree of intestinal mucosal damage(P<0.001,r=0.649),and a negative relation between SOD(r=-0.387),GSH-Px(r=-0.531)activities and degree of intestinal mucosal injury(P<0.001).Conclusion SDD protects against lipid peroxidation in intestinal tissues and reduces oxidative stress damage in rabbits with com -plete paraplegia.
Keywords：traumatic paraplegiaintestineselective decontamination of the digestive tractlipid peroxi-dationrabbits
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 121-125 )