The mechanism of mesenchymal stem cells conditional medium derived from human placenta repairing the acute kidney ischemia-reperfusion injury

WANG Juan; PAN Yong-jun; ZHANG Jie

doi:10.3969/j.issn.1009-4237.2017.12.011

Abstract：Objective To observe the protective effect of tail vein injection of human placenta mesenchymal stem cell conditional medium(MSCs-CM) repairing ischemia reperfusion injury (IRI).Methods Totally 30 healthy SD male rats were randomly divided into 3 groups,sham group,renal ischemia reperfusion model group (IR group) and MSCs conditional medium injection group (IR+MSCs group) with ten rats in each group.The rodent model of ische-mia-reperfusion ( IR) injury of kidney was established ,MSCs conditional medium were administrated into caudal vein at 24h after IR.The Cr in serum and urine with consecutive 4 days were detected ,and then kidney specimens were performed by PAS staining after rats were sacrificed ,DCFH-DA was used to detect the content of active oxygen in renal tissue,and the relative expression of HO-1 and NQO-1 were detected by Western Blot .Results Compared with IR group,the creatinine from serum and urine in MSCs treated group ( IR+MSCs group ) were ameliorated apparently after MSCs-CM injection .The pathological histology results demonstrated that the morphological damage of the renal tubule was observed in IR group,and amelioration was observed in MSCs treated group (IR+MSCs group).PAS showed that rats in the sham group had renal tubular injury ,which was more severe than that of the MSCs treated group(IR+MSCs group).Western Blot showed notably higher NAPDH quinone oxidoreductase 1(NQO1) and HO-1 protein expression ,the two indicators of anti-oxidative capacity ,in MSCs treated group ( IR+MSCs group ) than in sham group .Conclusion MSCs-CM transplanted after caudal vein is helpful for the repair of acute kidney injury induced by ischemia reperfusion injury .The repair mechanism might relate to the anti-oxidative stress .