Experimental study of acute lung injury in rabbits by underwater blast
WANG Qian-jin
YE Chang-qing
SHAO Xian-an
XU Chang-jiang
HUANG Yi-jiang
MA Hong-hao
HAO Jian
QI Xiao-lin
Abstract：Objective To establish a rabbit model of underwater blast-induced acute lung injury ( ubALI ) and to investigate the related mechanisms .Methods Thirty rabbits ( weighing ( 2.04 ±0.35 ) kg ) were randomly divided into two groups:control group (no injury) and experimental group(explode at 1.8 meters).The respiratory rate (RR) and arterial partial pressure of oxygen(PaO2) were assayed at 0,4,12,24h after injury.The expression of interleukin-8 (IL-8),tumor necrosis factor-α(TNF-α) in the bronchial alveolar lavage fluid (BALF) and blood samples were measured by ELISA .Morphological changes of tissues were detected by hematoxylin and eosin ( HE) staining,and pulmonary edema was determined by the wet-dry method.Results UbALI significantly increased RR at 0 and 4 h,and reduced PaO2 at 24 h(P<0.05).UbALI significantly increased the levels of IL-8 and TNF-αin both BALF and blood samples(P<0.05).The water content in the lung markedly increased ,and pulmonary edema and granulocyte infiltration were observed in lung tissues after ubALI .Conclusion These results demonstrate that we have created a reliable and reproducible ubALI model in rabbits .This model will be helpful for studying the mechanisms of ubALI and developing strategies for clinical treatment .
Keywords：lung injuryunderwater explosionanimal model
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 865-868 )