A novel mechanical stretch model in mice with superficial second degree burn

LI Fen; LI Wei; SHU Qin

doi:10.3969/j.issn.1009-4237.2017.08.011

Abstract：Objective To establish a novel mechanical stretch model in mice with superficial second degree burns,and to evaluate the stability and reliability of the model. Methods Sixty C57BL/6 mice aged 10-12 weeks were selected and randomly divided into 3 groups,including group A (mechanical stretch & superficial second degree burns),group B (sham operation & superficial second degree burns) and group C (normal).Seven sub-groups were set separately in group A and B according to different time points,with 4 mice in each group.After the skin was prepared,a 2 cm2 area of superficial second degree burn wound was established on mice back in group A and B at L1 level using YLS-5Q Desktop Scald Thermostatic Instrument.And the mechanical stretch was established on each mouse in group A by tissue defect suture method.The burn wound samples were taken for pathologic evaluation at 0,1,3,5,7,14d and 21d in group A and group B,as well as the skin in the same site of mice in group C.Results Superficial second degree burns were successfully built.According to pathologic evaluation results, the healing rate of wound in group A was significantly faster than that in group B.Group A had significantly larger wounds at 1d but had significantly smaller residual area at 5,7,14d than group B (P<0.05).The standard deviation was between 0.01 and 0.15,indicating that a stable and reliable mechanical stretch was sustainable until at least modeling day 14. Conclusion This study has successfully established a simple and scientific animal model of mechanical stretch stimulation with superficial second degree burn,which provides possible basis for studying the role of tension in scald wound.