Experimental research of effects of traction injury on the rat sciatic nerve
TONG Chang-jun
WEI You-xiu
HUANG Kang
Abstract：Objective To investigate the change of MRI , transforming growth factor β1(TGF-β1) and col-lagen type Ⅰ,Ⅲ( COL-Ⅰ,Ⅲ) after sciatic nerve traction injury .Methods Fifty adult male SD rats were ran-domly divided into group A and B .In group A ( the control group ) the sciatic nerve of rats was exposed only .In group B( the traction injury group ) the sciatic nerve of rats was tracted by smooth forceps to produce traction injury . Electron microscopy , immunochemistry , RT-PCR and Western Blot were performed to observe the morphological changes of the tracted nerve tissue and to determine the level of TGF-β1,COL-Ⅰand COL-Ⅲat 3 days and 2,4,6, 8 weeks after the surgery ,respectively .Results The injured nerve was characterized as thickened in size and in-creased T1 and T2 relaxation time.The expression of TGF-β1 in the traction injury group was significantly higher than that of the control group (P<0.05).In the meanwhile,the contents of COL-Ⅰ,COL-Ⅲwere positively corre-lated in a certain period(P<0.05).Conclusion Peripheral nerve fibrosis can be caused by nerve traction injury which induces increased expression of COL-Ⅰ,COL-Ⅲin the nerve .TGF-β1 is significantly higher than that of the control group ,which suggests that TGF-β1 plays an important role in the process of neural injury and fibrosis .
Keywords：traction injurysciatic nervesMRItransforming growth factorcollagen
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 35-38 )