Nerve growth factor-modified mesenchymal stem cells in the acceleration of recovery of inferior alveolar nerve injury in rabbit mandibular distraction osteogenesis
ZHAO Ying-hua
LIU Xiao-chang
GAO Le
CHEN Li-tong
YANG Zi-hui
WANG Lei
Abstract：Objective To study the effects of lentiviral-mediated human nerve growth factor beta ( hNGFβ) on repair of inferior alveolar nerve ( IAN) in mandibular distraction osteogenesis ( DO) in rabbits.Methods Bone marrow mesenchymal stem cells (MSCs) from rabbit mandibles were isolated and genetically engineered using re-combinant lentiviral vector containing hNGFβ. Twenty New Zealand white rabbits underwent mandibular DO and transplantation of 5 million MSCs transduced with hNGFβ-vector or control vector around the IAN in the bone frac-ture gap during the surgery(n=10).After the distraction,IAN samples were collected for histological and quantita-tive analysis at the 14th day of consolidation period.Results IAN histology showed that the experiment group had more regenerating nerve fibers and less nerve degeneration than the control group. Quantitative analysis of nerve morphology showed that the density of myelinated nerve fibers increased significantly compared with the control group.This indicated that the MSCs transduced with hNGFβcould significantly promote IAN repair during mandi-blar DO.Conclusion Lentiviral-mediated transduction with hNGFβin MSCs may provide an effective gene thera-py for reduction of nerve injury in mandibular DO clinically.
Keywords：nerve injurynerve growth factorgenemandibular bonedental alveolirabbit
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 168-171 )