Experimental study on TBI model in rats and the contents change of nerve growth factor and brain-derived neurotrophic factor of brain tissue
GUO Xin-rong
WANG Rui-hui
LI Na
WU Tao
Abstract：Objective To provide a preparation method of model with good repeatability and stable damage injury degree for the TBI experimental study.Methods Sixty-five SD rats were randomly divided into blank control group(Group A,intact),model group 1(Group B,blow speed 3m/s,blow depth 3mm),model group 2(Group C, blow speed 4m/s,blow depth 3mm),model group 3(Group D,blow speed 5m/s,blow depth 3mm) and sham-opera-tion group( Group E,skull opened without blow) .Different models of brain injury were produced by eCCI from dif-ferent variables.The model quality was evaluated through the contents of nerve growth factor( NGF) and brain-de-rived neurotrophic factor( BDNF) of brain tissue by immunohistochemical method.Results The NGF,BDNF in the brain tissue of cerebral cortex of neurons showed weak positive reaction in the blank control group and sham opera-tion group.The NGF,BDNF of damaged brain in three model groups showed positive reaction and the expression in-creased significantly with the increase of hit variables(P<0.01).Conclusion By eCCI,different appropriate TBI rat models can be prepared with the regulation of hit speed and depth.It is easily operated,controllable,and repeat-able and able to ideally cater to different individual needs.
Keywords：brain injurygrowth factornutrition factormodelrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 256-259 )