Effect of sivelestat sodium on plasma TNF-α,IL-6,IL-8 in rabbits after early blast lung injury

WANG Hai-long; YUAN Lei; LU Zhao-tong; ZHANG Bo

Abstract：Objective To investigate the effect of sivelestat sodium on plasma TNF-α,IL-6,IL-8 in rabbits after early lung injury.Methods Totally 30 healthy New Zealand rabbits were randomly divided into 2 groups:group A was the control group with 15 rabbits;B group was the treatment group ( sivelestat sodium, Sweden, CAS 150374-95-1) with 15 rabbits.Detonator explosion was employed to make rabbit blast lung injury models.Siveles-tat sodium(10mg/kg) was given through ear vein injection immediately after injury in group B;saline of the same dose was given through intravenous injection in group A.The content changes of TNF-α,IL-6,IL-8 at the time pha-ses of 1,6,12h and 24h after injury in plasma were measured by ELISA.Lung tissue of the rabbits was dissected and paraffin section was made.The pathological changes were observed after HE staining.Results The levels of TNF-α,IL-6,IL-8 in plasma in group B were significantly decreased compared with group A after treatment at time phases of 1,6 and 12h.The condition of edema and hemorrhage of lung tissue in group B was significantly relieved compared with group A.Conclusion Sivelestat sodium can reduce the content of TNF-α,IL-6,IL-8 in plasma after lung blast injury and inhibit consequent inflammatory reaction,thereby reducing damage of blast injury to the lung.