Experimental study of isoliquiritigenin neuroprotection on traumatic brain injury in rats

JIANG Tuo-zhi; YANG Yong-ming; LI Zhi-yun

Abstract：Objective To observe the effect of isoliquiritigenin ( ISL ) on pathological changes and SOD , MDA,IL-6,TNF-αand water content of brain tissue after traumatic brain injury in rats and to investigate its possible mechanism for brain rehabilitation .Methods Rats were randomly divided into 3 groups: sham group,TBI group and ISL group.TBI model was established with the modified Feeney's method.The ISL group received intragastric-ly ISL 20mg/kg 1 times/d,the TBI group and sham group received the same amount of physiological gavage 2 hours after surgery .After 7 days rats were sacrificed to measure the SOD ,MDA of brain tissue using endpoint method ,and ELISA was employed to detect the content of IL-6 and TNF-α,wet-dry weighting method was employed to measure water content in the brain tissue .The pathology of the brain tissues was observed under optical microscope through HE staining.Results Compared with TBI group ,the content of MDA was decreased ,the content of SOD ,IL-6 and TNF-αwas increased ,and the pathology of the brain tissues was improved .Conclusion ISL can promote brain tis-sue rehabilitation of traumatic brain injury in rats .The underlying mechanism may be associated with reduction of oxidative stress and regulation of cytokines of brain tissue .