A study of transfection efficiency by NF-кB decoy dsODNs/carrier in the alveolar macrophages in vito during acute lung injury

YIN Wen; WANG Qian-mei; FENG Yang; ZHU Jin-wen; LI Ming-yue; GAO Lu

Abstract：Objective To study the transfection efficiency by NF -кB decoy dsODNs/carrier in the alveo-lar macrophages in vito during acute lung injury by NF -кB decoy dsODNs/liposome technology .Intended results will provide a preliminary experimental basis in vitro .It could provide the feasibe experiment basis for screening drug targets and seek dfficient and reasonable treatment to prevent and cure SIRS /ALI.Methods Animal model of hemorrhagic shock caused acute lung injury was established .Different stimulation and complex processing were giv-en to alveolar macrophages in vitro using Lipofectamine 2000 as carrier.The transfection efficiency of each phase and the best time to determine dfficient transfection was measured .The LDH level trasformation by different pre-treatment were detected in different time points .Results When NF-кB decoy dsODNs/Lipofectamine2000 rate was 1∶5 and transfected time was six hours ,the transfection efficiency was highest ,and the cytotoxicity was moder-ate.Conclusion NF-кB decoy dsODNs could significantly improve the transfection efficiency within a proper dsODNs/Lipofectamine2000 rate and reduce injury of alveolar macrophages by different stimulations as much as pos -sible.Decoy dsODNs strategy provides new ideas and approaches for preventing and treating for SIRS /ALI, deter-mines the precise target and screening specific inflammatory therapy for the clinical treatment , and determines the precise target of the clinical drug development .