Effect of neurolytic celiac plexus block on hepatocellular apoptosis after partial hepatectomy in rats

LI Jun; CHE Jian-xiang; WEI Xiao-hong; QIU Qing-ming; LIN Lu; BAI Shu-rong; PAN Fan; SUN Xiao-qin

Abstract：Objective To investigate the effects of neurolytic celiac plexus block ( NCPB) on the apoptosis of residual liver cells after partial hepatectomy ( PH) in rats.Methods Sixty clean male SD rats were randomly di-vided into control group and NCPB group after 70%PH.NCPB group received pertaneous neurolytic celiac plexus block with 0.5%lidocaine(one time/day),and control group received the same procedures , but physiological saline was injected instead of 0.5%lidocaine.The residual liver function , liver blood flow and expressions of apoptosis-related protein BAX and Bcl-2 were determined at 1 day,3 days and 7 days after PH.Results The aspartate amin-otransferase(AST) and alanine aminotransferase (ALT) content of PH rats in the NCPB group was significantly low-er than that of rats in control group at 1 day,3 days and 7 days (P<0.01 or P<0.05).The hepatocytes showed more steatosis,and spotty or patchy necrosis after PH in the control group than in the NCPB group .At all the re-spective time points ,the liver blood flow of the NCPB group was higher than that of the control group ( P<0.01 ) . The hepatic BAX expressions were gradually decreased in the two groups after PH , but at the corresponding time point it was significantly lower in NCPB group than that of the control group (P<0.05 or P<0.01).The Bcl-2 ex-pressions were increased significantly ,which was remarkably higher in the NCPB group than that in the control group (P<0.01).At 3 days and 7 days,the BAX/Bcl-2 ratio was significantly lower in NCPB group than that in the con-trol group(P<0.01).Conclusion NCPB treatment can alleviate the early damage of liver function after PH in rats,which may be associated with the inhibition of hepatocellular apoptosis .