Establishment of a rat model of acute lung injury following chest impact injury combined with LPS challenge
WANG Hai-yan
DU Juan
ZHAO Yu-jin
LI Hai-sheng
YANG Ce
YUE Cai-li
JIANG Jian-xin
Abstract：Objective To establish an animal model of acute lung injury ( ALI) following chest impact combined with lipopolysaccharide ( LPS) challenge .Methods The lung injury of rats was caused by the BMI-Ⅲbiological impact device on right lung at quasi-static pattern .Using 250 kPa as a suitable driving pressure ,the chest injured rats were injected with 10,15,20 mg/kg LPS via tail vein respectively .The outcome of the ALI rats was ob-served.Results The driving pressure of 250 kPa induced moderate lung injury ,no animal died after experiment . The mortality of chest injury rats with 20mg/kg of LPS was 80% within 48 hours,70% with 15mg/kg of LPS,and 20%with 10mg/kg of LPS.We set up the ALI model by 250 kPa driving pressure combined with 15mg/kg LPS. The biopsy features of the lungs demonstrated typical pathological changes after ALI .Conclusion 250 kPa driving pressure combined with 15mg/kg LPS is an ideal injury condition for ensuring a successful injury model .The estab-lished model of ALI is relatively simple ,easily controllable and highly repeatable ,which can be used as a feasible model for the study of ALI .
Keywords：chest injurylung injuryimpact injurybiological impact machinerat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 46-49 )